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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Streptomyces coelicolor with promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660, time series

ABSTRACT: The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Tina Boussard

PROVIDER: E-SMDB-2244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reverse engineering of industrial pharmaceutical-producing actinomycete strains using DNA microarrays.

Lum Amy M AM Huang Jianqiang J Hutchinson C Richard CR Kao Camilla M CM

Metabolic engineering 20040701 3

Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56- ...[more]

PMID: 15256208

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