Transcription profiling of Streptomyces coelicolor with promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660, time series

ABSTRACT: The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Tina Boussard 

PROVIDER: E-SMDB-2244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress