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Transcription profiling of mouse liver and spleen from strains that are susceptible and tolerant to trypanosome infection

ABSTRACT: Tissues were harvested from mice from two congenic strains (Tir1CC and Tir3CC) and their two control strains (Tir1AA and Tir3AA). The congenic strains were bred from inbred lines known to be susceptible (A/J) and tolerant (C57BL/6) to Trypanosome infection, such that C57BL/6 genomic regions underlying QTL for trypanotolerance were present in an A/J genetic background. Liver and spleen tissues were harvested. For each strain RNA was prepared from various tissues of twenty mice. For each condition, RNA extracts from individuals were pooled (5 per pool) prior to hybridisation.

ORGANISM(S): Mus musculus

SUBMITTER: Susan Anderson

PROVIDER: E-TABM-865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genotype and expression analysis of two inbred mouse strains and two derived congenic strains suggest that most gene expression is trans regulated and sensitive to genetic background.

Noyes Harry A HA Agaba Morris M Anderson Susan S Archibald Alan L AL Brass Andy A Gibson John J Hall Laurence L Hulme Helen H Oh Sung Jong SJ Kemp Stephen S

BMC genomics 20100607

<h4>Background</h4>Differences in gene expression may be caused by nearby DNA polymorphisms (cis regulation) or by interactions of gene control regions with polymorphic transcription factors (trans regulation). Trans acting loci are much harder to detect than cis acting loci and their effects are much more sensitive to genetic background.<h4>Results</h4>To quantify cis and trans regulation we correlated haplotype data with gene expression in two inbred mouse strains and two derived congenic line ...[more]

PMID: 20529291

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