Dataset Information
Transcription profiling of human haematological neoplasias from blood samples and cell lines
Ontology highlight
ABSTRACT:
Two haematological neoplasia cell lines: U937 (promonocytic lymphoma) versus Jurkat (T leukemia).
Different types of haematological neoplasias: healthy donors, B-CLL patients, myeloid leukemia patients and U937 and Jurkat cell cultures samples.
Two hematological neoplasia with different origin (lymphoid for B-CLL and myeloid for the myeloid group).
Two types of myeloid leukemias (Acute myeloid leukemia and myelodisplastic syndrome).
B-CLL patients with different clinical behaviour.
Healthy donors and B-CLL patients.
Normalization was performed using two different methods, robust quantile normalization (QRN) and variance stabilization normalization (VSN), so that a set of statistcially significant probes found using both methods could be obtained.
ORGANISM(S): Homo sapiens
DISEASE(S): B-cell chronic lymphocytic leukemia
SUBMITTER: Patricia Álvarez
PROVIDER: E-TABM-87 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transcriptional profiling of hematologic malignancies with a low-density DNA microarray.
Clinical chemistry 20061221 2
<h4>Background</h4>High-density microarrays are powerful tools for expression analysis of thousands of genes simultaneously; however, experience with low-density microarrays in gene expression studies has been limited.<h4>Methods</h4>We developed an optimized procedure for gene expression analysis based on a microarray containing 538 oligonucleotides and used this procedure to analyze neoplastic cell lines and whole-blood samples from healthy individuals and patients with different hematologic n ...[more]
