Characterization of proteome profile data of chemicals based on data-independent acquisition MS with SWATH method.

ABSTRACT: Transcriptomic data of cultured cells treated with a chemical are widely recognized as useful numeric information that describes the effects of the chemical. This property is due to the high coverage and low arbitrariness of the transcriptomic data as profiles of chemicals. Considering the importance of posttranslational regulation, proteomic profiles could provide insights into the unrecognized aspects of the effects of chemicals. Therefore, this study aimed to address the question of how well the proteomic profiles obtained using data-independent acquisition (DIA) with the sequential window acquisition of all theoretical mass spectra, which can achieve comprehensive and arbitrariness-free protein quantification, can describe chemical effects. We demonstrated that the proteomic data obtai

SUBMITTER: Ishiguro H 

PROVIDER: S-EPMC10006730 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature