A generalizable nanopore sensor for highly specific protein detection at single-molecule precision.

ABSTRACT: Protein detection has wide-ranging implications in molecular diagnostics. Substantial progress has been made in protein analytics using nanopores and the resistive-pulse technique. Yet, a long-standing challenge is implementing specific interfaces for detecting proteins without the steric hindrance of the pore interior. Here, we formulate a class of sensing elements made of a programmable antibody-mimetic binder fused to a monomeric protein nanopore. This way, such a modular design significantly expands the utility of nanopore sensors to numerous proteins while preserving their architecture, specificity, and sensitivity. We prove the power of this approach by developing and validating nanopore sensors for protein analytes that drastically vary in size, charge, and structural complexity. Th

SUBMITTER: Ahmad M 

PROVIDER: S-EPMC10027671 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature