A decrease in NR2B expression mediated by DNA hypermethylation induces perioperative neurocognitive disorder in aged mice.

ABSTRACT:

Aims

This study was designed to investigate the role of NR2B and the contribution of DNA methylation to NR2B expression in the pathogenesis of PND.

Methods

Eighteen-month-old C57BL/6J mice were subjected to experimental laparotomy under 1.4% isoflurane anesthesia. Hippocampus-dependent learning and memory were evaluated by using the Barnes maze and contextual fear conditioning tests. The protein and mRNA expression levels of NR2B were evaluated by western blotting and qRT-PCR respectively, and the methylation of the NR2B gene was examined by using targeted bisulfite sequencing. Long-term synaptic plasticity (LTP) was measured by electrophysiology.

Results

Mice that underwent laparotomy exhibited hippocampus-dependent cognitive deficits accompanied by decreased NR2B ex

SUBMITTER: Xu F 

PROVIDER: S-EPMC10068472 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature