Pericyte stem cells induce Ly6G⁺ cell accumulation and immunotherapy resistance in pancreatic cancer.

ABSTRACT: We report the identification of a cell population that shares pericyte, stromal and stemness features, does not harbor the Kras^G12D mutation and drives tumoral growth in vitro and in vivo. We term these cells pericyte stem cells (PeSCs) and define them as CD45^- EPCAM^- CD29⁺ CD106⁺ CD24⁺ CD44⁺ cells. We perform studies with p48-Cre;Kras^G12D (KC), pdx1-Cre;Kras^G12D ;Ink4a/Arf^fl/fl (KIC) and pdx1-Cre;Kras^G12D ;p53^R172H (KPC) and tumor tissues from PDAC and chronic pancreatitis patients. We also perform single-cell RNAseq analysis and reveal a unique signature of PeSC. Under steady-state conditions, PeSCs are barely detectable in the pancreas but present in the ne

SUBMITTER: Wu Z 

PROVIDER: S-EPMC10074138 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature