Substrate Induced Movement of the Metal Cofactor between Active and Resting State.
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ABSTRACT:
SUBMITTER: Marsden SR
PROVIDER: S-EPMC10099721 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
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Substrate Induced Movement of the Metal Cofactor between Active and Resting State.
Marsden Stefan R SR Wijma Hein J HJ Mohr Michael K F MKF Justo Inês I Hagedoorn Peter-Leon PL Laustsen Jesper J Jeffries Cy M CM Svergun Dmitri D Mestrom Luuk L McMillan Duncan G G DGG Bento Isabel I Hanefeld Ulf U
Angewandte Chemie (International ed. in English) 20221109 49
Regulation of enzyme activity is vital for living organisms. In metalloenzymes, far-reaching rearrangements of the protein scaffold are generally required to tune the metal cofactor's properties by allosteric regulation. Here structural analysis of hydroxyketoacid aldolase from Sphingomonas wittichii RW1 (SwHKA) revealed a dynamic movement of the metal cofactor between two coordination spheres without protein scaffold rearrangements. In its resting state configuration (M<sup>2+</sup> <sub>R</sub ...[more]
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