Novel genetic regulators of fibrinogen synthesis identified by an in vitro experimental platform.

ABSTRACT:

Background

Fibrinogen has an established, essential role in both coagulation and inflammatory pathways, and these processes are deeply intertwined in the development of thrombotic and atherosclerotic diseases. Previous studies aimed to better understand the (patho) physiological actions of fibrinogen by characterizing the genomic contribution to circulating fibrinogen levels.

Objectives

Establish an in vitro approach to define functional roles between genes within these loci and fibrinogen synthesis.

Methods

Candidate genes were selected on the basis of their proximity to genetic variants associated with fibrinogen levels and expression in hepatocytes and HepG2 cells. HepG2 cells were transfected with small interfering RNAs targeting candidate genes and cultured in th

SUBMITTER: Dobson DA 

PROVIDER: S-EPMC10111212 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature