Novel regulators of islet function identified from genetic variation in mouse islet Ca²⁺ oscillations.

ABSTRACT: Insufficient insulin secretion to meet metabolic demand results in diabetes. The intracellular flux of Ca²⁺ into β-cells triggers insulin release. Since genetics strongly influences variation in islet secretory responses, we surveyed islet Ca²⁺ dynamics in eight genetically diverse mouse strains. We found high strain variation in response to four conditions: (1) 8 mM glucose; (2) 8 mM glucose plus amino acids; (3) 8 mM glucose, amino acids, plus 10 nM glucose-dependent insulinotropic polypeptide (GIP); and (4) 2 mM glucose. These stimuli interrogate β-cell function, α- to β-cell signaling, and incretin responses. We then correlated components of the Ca²⁺ waveforms to islet protein abundances in the same strains used for the Ca²⁺ measurements. To

SUBMITTER: Emfinger CH 

PROVIDER: S-EPMC10547476 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature