Metagenomic next-generation sequencing and proteomics analysis in pediatric viral encephalitis and meningitis.

ABSTRACT:

Introduction

Early and accurate identification of pathogens is essential for improved outcomes in patients with viral encephalitis (VE) and/or viral meningitis (VM).

Methods

In our research, Metagenomic next-generation sequencing (mNGS) which can identify viral pathogens unbiasedly was performed on RNA and DNA to identify potential pathogens in cerebrospinal fluid (CSF) samples from 50 pediatric patients with suspected VEs and/or VMs. Then we performed proteomics analysis on the 14 HEV-positive CSF samples and another 12 CSF samples from health controls (HCs). A supervised partial least squaresdiscriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) model was performed using proteomics data.

Results

Ten viruses in 48% patients were identified and the most commo

SUBMITTER: Wang YL 

PROVIDER: S-EPMC10161730 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature