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Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy.

ABSTRACT: The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1^+/GFP mice. This study's method was effective in elucidating inner ear function under both physiological and pathological conditions.

SUBMITTER: Urata S

PROVIDER: S-EPMC10256671 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy.

Urata Shinji S Okabe Shigeo S

Anatomical science international 20230211 3

The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1<sup>+/GFP</sup> mice. This study's method was effective in elucidating inner ear function under both phys ...[more]

PMID: 36773194

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