Clinical significance of B7-H3 expression in circulating CD4⁺CD25^high T cells, CD14⁺ monocytes, and plasma for the progression of HIV infection.

ABSTRACT:

Background

B7-H3 is an important immune checkpoint molecule that plays a negative role in immune regulation. This study was aimed to explore B7-H3 expression in HIV-infected patients and its clinical significance.

Methods

To explore the expression and clinical significance of B7-H3 in HIV-infected patients, we investigated the B7-H3 expression pattern and the correlation of B7-H3 expression with clinical parameters of HIV-infected patients with different levels of CD4⁺ T cells. To assess the role of B7-H3 in regulating the function of T cells in HIV infection, we performed a proliferation assay and T cell function test in vitro.

Results

B7-H3 expression in HIV-infected patients was significantly higher than that in healthy controls. mB7-H3 expression on CD4⁺CD25^high T cells and CD14⁺ monocytes increased with disease progression. mB7-H3 expression on CD4⁺CD25^high T cells and monocytes was negatively correlated with lymphocyte count, CD4⁺T cell count, and positively correlated with HIV viral load in HIV-infected patients. when the number of CD4⁺ T cells in HIV-infected patients was ≥ 200/µL, sB7-H3 and mB7-H3 expression levels on CD4⁺CD25^high T cells and monocytes were negatively correlated with lymphocyte count, CD4⁺T cell count. sB7-H3 and mB7-H3 expression on monocytes were positively correlated with HIV viral load. B7-H3 inhibited the proliferation of lymphocytes and the secretion of IFN-γ in vitro, especially the ability of CD8⁺ T cells to secrete IFN-γ.

Conclusions

B7-H3 played an important negative regulatory role in anti-HIV infection immunity. It could be used as a potential biomarker for the progression of HIV infection and a novel target for the treatment of HIV infection.

SUBMITTER: Xu JC 

PROVIDER: S-EPMC10334634 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature