Alternative polyadenylation factor CPSF6 regulates temperature compensation of the mammalian circadian clock.

ABSTRACT: A defining property of circadian clocks is temperature compensation, characterized by the resilience of their near 24-hour free-running periods against changes in environmental temperature within the physiological range. While temperature compensation is evolutionary conserved across different taxa of life and has been studied within many model organisms, its molecular underpinnings remain elusive. Posttranscriptional regulations such as temperature-sensitive alternative splicing or phosphorylation have been described as underlying reactions. Here, we show that knockdown of cleavage and polyadenylation specificity factor subunit 6 (CPSF6), a key regulator of 3'-end cleavage and polyadenylation, significantly alters circadian temperature compensation in human U-2 OS cells. We apply a combin

SUBMITTER: Schmal C 

PROVIDER: S-EPMC10335657 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature