PRKRA promotes pancreatic cancer progression by upregulating MMP1 transcription via the NF-κB pathway.

ABSTRACT:

Objective

Pancreatic cancer (PC) is highly malignant, but the underlying mechanisms of cancer progression remain unclear. PRKRA is involved in cellular stress response, but its role in PC was unknown.

Methods

The expression of PRKRA between normal and tumor tissues were compared, and the prognostic value of PRKRA was evaluated. SiRNA and plasmids were applied to investigate the effects of PRKRA on PC cells. Organoids and cell lines with knockout and overexpression of PRKRA were established by CRISPR/Cas9 and lentivirus. The effects of PRKRA on PC were evaluated in vivo by cell-derived xenografts. The downstream genes of PRKRA were screened by transcriptome sequencing. The regulation of the target gene was validated by RT-qPCR, western blot, ChIP and dual luciferase reporter

SUBMITTER: Qiu J 

PROVIDER: S-EPMC10361375 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature