The formation of K_V2.1 macro-clusters is required for sex-specific differences in L-type Ca_V1.2 clustering and function in arterial myocytes.

ABSTRACT: In arterial myocytes, the canonical function of voltage-gated Ca_V1.2 and K_V2.1 channels is to induce myocyte contraction and relaxation through their responses to membrane depolarization, respectively. Paradoxically, K_V2.1 also plays a sex-specific role by promoting the clustering and activity of Ca_V1.2 channels. However, the impact of K_V2.1 protein organization on Ca_V1.2 function remains poorly understood. We discovered that K_V2.1 forms micro-clusters, which can transform into large macro-clusters when a critical clustering site (S590) in the channel is phosphorylated in arterial myocytes. Notably, female myocytes exhibit greater phosphorylation of S590, and macro-cluster formation compared to males. Contrary to

SUBMITTER: Matsumoto C 

PROVIDER: S-EPMC10371172 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature