Establishment of a Novel Anti-CD44 Variant 10 Monoclonal Antibody C₄₄Mab-18 for Immunohistochemical Analysis against Oral Squamous Cell Carcinomas.

ABSTRACT: Head and neck squamous cell carcinoma (HNSCC) is the most common type of head and neck cancer, and has been revealed as the second-highest expression of CD44 in cancers. CD44 has been investigated as a cancer stem cell marker of HNSCC and plays a critical role in tumor malignant progression. Especially, splicing variant isoforms of CD44 (CD44v) are overexpressed in cancers and considered a promising target for cancer diagnosis and therapy. We developed monoclonal antibodies (mAbs) against CD44 by immunizing mice with CD44v3-10-overexpressed PANC-1 cells. Among the established clones, C₄₄Mab-18 (IgM, kappa) reacted with CHO/CD44v3-10, but not with CHO/CD44s and parental CHO-K1 using flow cytometry. The epitope mapping using peptides that cover variant exon-encoded regions reveale

SUBMITTER: Ishikawa K 

PROVIDER: S-EPMC10378409 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature