Novel insights into genetic characteristics of bla_GES-encoding plasmids from hospital sewage.

ABSTRACT:

Introduction

The prevalence of Guiana extended-spectrum (GES)-type carbapenemase producers is increasing worldwide, and hospital water environments are considered as potential reservoirs. However, the genetic features underlying this resistance are not yet fully understood. This study aimed to characterize bla_GES-encoding plasmids from a single-hospital sewage sample in Japan.

Methods

Carbapenemase producers were screened using carbapenemase-selective agar and polymerase chain reaction. Whole-genome sequencing analyzes were performed on the carbapenemase-producing isolates.

Results

Eleven gram-negative bacteria (four Enterobacter spp., three Klebsiella spp., three Aeromonas spp., and one Serratia spp.) with bla

SUBMITTER: Ota Y 

PROVIDER: S-EPMC10469963 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature