Stability and genetic insights of the co-existence of bla_CTX-M-65, bla_OXA-1, and mcr-1.1 harboring conjugative IncI2 plasmid isolated from a clinical extensively-drug resistant Escherichia coli ST744 in Shanghai.

ABSTRACT:

Background

Co-existence of colistin, β-lactam and carbapenem in multidrug-resistant Enterobacteriaceae isolates poses a serious threat to public health. In this study, we investigated and characterized the co-occurrence of bla_CTX-M-65, bla_OXA-1, and mcr-1.1 strain isolated from a clinical extensively-drug-resistant Escherichia coli ST744 in Shanghai.

Methods

Antimicrobial susceptibility test was carried out by agar dilution methods. Whole genome sequencing was conducted, and resistance genes, and sequence types of colistin in E. coli isolates were analyzed. Plasmid stability and amino acid mutations were assessed in E. coli isolates.

Results

A colistin resistant E. coli ST744, named ECPX221,

SUBMITTER: Feng J 

PROVIDER: S-EPMC10481164 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature