Electrophysiological and morphological characterization of single neurons in intact human brain organoids.

ABSTRACT: Brain organoids represent a new model system for studying developmental human neurophysiology. Methods for studying the electrophysiology and morphology of single neurons in organoids require acute slices or dissociated cultures. While these methods have advantages (e.g., visual access, ease of experimentation), they risk damaging cells and circuits present in the intact organoid. To access single cells within intact organoid circuits, we have demonstrated a method for fixturing and performing whole cell patch clamp recording from intact brain organoids using both manual and automated tools. We demonstrate applied electrophysiology methods development followed by an integration of electrophysiology with reconstructing the morphology of the neurons within the brain organoid using dye fillin

SUBMITTER: Landry CR 

PROVIDER: S-EPMC10483933 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature