Imaging of fibrogenesis in the liver by [¹⁸F]TZ-Z09591, an Affibody molecule targeting platelet derived growth factor receptor β.

ABSTRACT:

Background

Platelet-derived growth factor receptor beta (PDGFRβ) is a receptor overexpressed on activated hepatic stellate cells (aHSCs). Positron emission tomography (PET) imaging of PDGFRβ could potentially allow the quantification of fibrogenesis in fibrotic livers. This study aims to evaluate a fluorine-18 radiolabeled Affibody molecule ([¹⁸F]TZ-Z09591) as a PET tracer for imaging liver fibrogenesis.

Results

In vitro specificity studies demonstrated that the trans-Cyclooctenes (TCO) conjugated Z09591 Affibody molecule had a picomolar affinity for human PDGFRβ. Biodistribution performed on healthy rats showed rapid clearance of [¹⁸F]TZ-Z09591 through the kidneys and low liver background uptake. Autoradiography (ARG) studies on fibrotic livers from mi

SUBMITTER: Wegrzyniak O 

PROVIDER: S-EPMC10513984 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature