Developmental progression of the nasopharyngeal microbiome during childhood and association with the lower airway microbiome.

ABSTRACT:

Background

The upper (URT) and lower (LRT) respiratory tract feature distinct environments and responses affecting microbial colonization but investigating the relationship between them is technically challenging. We aimed to identify relationships between taxa colonizing the URT and LRT and explore their relationship with development during childhood.

Methods

We employed V4 16S rDNA sequencing to profile nasopharyngeal swabs and tracheal aspirates collected from 183 subjects between 20 weeks and 18 years of age. These samples were collected prior to elective procedures at the Children's Hospital of Philadelphia over the course of 20 weeks in 2020, from otherwise healthy subjects enrolled in a study investigating potential reservoirs of SARS-CoV-2.

Findings

After extr

SUBMITTER: Hernandez-Leyva AJ 

PROVIDER: S-EPMC10543049 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature