Mettl14-mediated m⁶A modification ensures the cell-cycle progression of late-born retinal progenitor cells.

ABSTRACT: Neural progenitor cells lengthen their cell cycle to prime themselves for differentiation as development proceeds. It is currently not clear how they counter this lengthening and avoid being halted in the cell cycle. We show that N⁶-methyladenosine (m⁶A) methylation of cell-cycle-related mRNAs ensures the proper cell-cycle progression of late-born retinal progenitor cells (RPCs), which are born toward the end of retinogenesis and have long cell-cycle length. Conditional deletion of Mettl14, which is required for depositing m⁶A, led to delayed cell-cycle exit of late-born RPCs but has no effect on retinal development prior to birth. m⁶A sequencing and single-cell transcriptomics revealed that mRNAs involved in elongating the cell cycle were highly

SUBMITTER: Li L 

PROVIDER: S-EPMC10543643 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature