Dataset Information

Quantitative assessment of angioplasty-induced vascular inflammation with ¹⁹F cardiovascular magnetic resonance imaging.

ABSTRACT:

Background

Macrophages play a pivotal role in vascular inflammation and predict cardiovascular complications. Fluorine-19 magnetic resonance imaging (¹⁹F MRI) with intravenously applied perfluorocarbon allows a background-free direct quantification of macrophage abundance in experimental vascular disease models in mice. Recently, perfluorooctyl bromide-nanoemulsion (PFOB-NE) was applied to effectively image macrophage infiltration in a pig model of myocardial infarction using clinical MRI scanners. In the present proof-of-concept approach, we aimed to non-invasively image monocyte/macrophage infiltration in response to carotid artery angioplasty in pigs using ¹⁹F MRI to assess early inflammatory response to mechanical injury.

Methods

In eight minipigs, two different types of vascular injury were conducted: a mild injury employing balloon oversize angioplasty only (BA, n = 4) and a severe injury provoked by BA in combination with endothelial denudation (BA + ECDN, n = 4). PFOB-NE was administered intravenously three days after injury followed by ¹H and ¹⁹F MRI to assess vascular inflammatory burden at day six. Vascular response to mechanical injury was validated using X-ray angiography, intravascular ultrasound and immunohistology in at least 10 segments per carotid artery.

Results

Angioplasty was successfully induced in all eight pigs. Response to injury was characterized by positive remodeling with predominantly adventitial wall thickening and concomitant infiltration of monocytes/macrophages. No severe adverse reactions were observed following PFOB-NE administration. In vivo ¹⁹F signals were only detected in the four pigs following BA + ECDN with a robust signal-to-noise ratio (SNR) of 14.7 ± 4.8. Ex vivo analysis revealed a linear correlation of ¹⁹F SNR to local monocyte/macrophage cell density. Minimum detection limit of infiltrated monocytes/macrophages was estimated at approximately 410 cells/mm².

Conclusions

In this proof-of-concept study, ¹⁹F MRI enabled quantification of monocyte/macrophage infiltration after vascular injury with sufficient sensitivity. This may provide the opportunity to non-invasively monitor vascular inflammation with MRI in patients after angioplasty or even in atherosclerotic plaques.

SUBMITTER: Nienhaus F

PROVIDER: S-EPMC10546783 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Publications

Quantitative assessment of angioplasty-induced vascular inflammation with <sup>19</sup>F cardiovascular magnetic resonance imaging.

Nienhaus Fabian F Walz Moritz M Rothe Maik M Jahn Annika A Pfeiler Susanne S Busch Lucas L Stern Manuel M Heiss Christian C Vornholz Lilian L Cames Sandra S Cramer Mareike M Schrauwen-Hinderling Vera V Gerdes Norbert N Temme Sebastian S Roden Michael M Flögel Ulrich U Kelm Malte M Bönner Florian F

Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance 20231003 1

<h4>Background</h4>Macrophages play a pivotal role in vascular inflammation and predict cardiovascular complications. Fluorine-19 magnetic resonance imaging (<sup>19</sup>F MRI) with intravenously applied perfluorocarbon allows a background-free direct quantification of macrophage abundance in experimental vascular disease models in mice. Recently, perfluorooctyl bromide-nanoemulsion (PFOB-NE) was applied to effectively image macrophage infiltration in a pig model of myocardial infarction using ...[more]

PMID: 37784080

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Dataset Information

Quantitative assessment of angioplasty-induced vascular inflammation with ¹⁹F cardiovascular magnetic resonance imaging.

Background

Methods

Results

Conclusions

Publications

Quantitative assessment of angioplasty-induced vascular inflammation with <sup>19</sup>F cardiovascular magnetic resonance imaging.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Quantitative assessment of angioplasty-induced vascular inflammation with 19F cardiovascular magnetic resonance imaging.

Background

Methods

Results

Conclusions

Publications

Quantitative assessment of angioplasty-induced vascular inflammation with <sup>19</sup>F cardiovascular magnetic resonance imaging.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Quantitative assessment of angioplasty-induced vascular inflammation with ¹⁹F cardiovascular magnetic resonance imaging.