Tetrameric Fluorescent Antigen Arrays for Single-Step Identification of Antigen-Specific B Cells.

ABSTRACT: Fluorescent antigen production is a critical step in the identification of antigen-specific B cells. Here, we detailed the preparation, purification, and the use of four-arm, fluorescent PEG-antigen conjugates to selectively identify antigen-specific B cells through avid engagement with cognate B cell receptors. Using modular click chemistry and commercially available fluorophore kit chemistries, we demonstrated the versatility of preparing customized fluorescent PEG-conjugates by creating distinct arrays for proteolipid protein (PLP139-151) and insulin, which are important autoantigens in murine models of multiple sclerosis and type 1 diabetes, respectively. Assays were developed for each fluorescent conjugate in its respective disease model using flow cytometry. Antigen arrays were compa

SUBMITTER: Apley KD 

PROVIDER: S-EPMC10604357 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature