Genome-wide transcription and repair maps of Caenorhabditis elegans.

ABSTRACT: We have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts in the Caenorhabditis elegans (C. elegans) genome. We focus on the C. elegans ortholog of the human XPC-deficient strain (xpc-1) and its exclusive use of transcription-coupled repair. We provide evidence demonstrating the utility of xpc-1 XR-seq as a remarkable tool for detecting nascent transcription and identifying new transcripts. The integration of epigenetic markers, chromatin states, enhancer RNA and long intergenic non-coding RNA annotations supports the robust detection of intergenic nascent transcription by XR-seq. Overall, our results provide a comprehensive view of the transcription-coupled repair landscape in C. elegans, highlighting its potential contributions to our understanding of DNA repair mechanisms and non-coding RNA biology.

SUBMITTER: Kose C 

PROVIDER: S-EPMC10614815 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature