Dataset Information

Calcium electroporation of esophageal cancer induces gene expression changes: a sub-study of a phase I clinical trial.

ABSTRACT:

Purpose

In this study, we aim to investigate gene expression changes in tumor samples obtained from patients with esophageal cancer treated with calcium electroporation. Previously, local treatment with calcium electroporation has been shown to induce gene expression alterations, potentially contributing to a more tumor-hostile microenvironment.

Methods

In this sub-study of a phase I clinical trial, we included five patients with esophageal cancer treated with calcium electroporation. We compared cancer-associated gene expression patterns in tumor samples before and after treatment. Furthermore, we used linear support vector regression to predict the cellular composition of tumor samples.

Results

Using differential expression analysis, we identified the downregulation of CXCL14 and upregulation of CCL21, ANGPTL4, and CRABP2 genes. We also found a decreased predicted proportion of dendritic cells while the proportion of neutrophils was increased.

Conclusion

This study provides evidence that calcium electroporation for esophageal cancer induces local transcriptional changes and possibly alters the cellular composition of the tumor microenvironment. The results are explorative, larger studies are needed to confirm and further correlate our findings with clinical outcomes.

SUBMITTER: Egeland C

PROVIDER: S-EPMC10620256 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Publications

Calcium electroporation of esophageal cancer induces gene expression changes: a sub-study of a phase I clinical trial.

Egeland Charlotte C Balsevicius Lukas L Gögenur Ismail I Gehl Julie J Baeksgaard Lene L Garbyal Rajendra Singh RS Achiam Michael Patrick MP

Journal of cancer research and clinical oncology 20230909 17

<h4>Purpose</h4>In this study, we aim to investigate gene expression changes in tumor samples obtained from patients with esophageal cancer treated with calcium electroporation. Previously, local treatment with calcium electroporation has been shown to induce gene expression alterations, potentially contributing to a more tumor-hostile microenvironment.<h4>Methods</h4>In this sub-study of a phase I clinical trial, we included five patients with esophageal cancer treated with calcium electroporat ...[more]

PMID: 37688629

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Project description:IntroductionSkin malignancy is a distressing problem for many patients, and clinical management is challenging. This article describes the protocol for the Calcium Electroporation Response Study (CaEP-R) designed to investigate tumour response to calcium electroporation and is a descriptive guide to calcium electroporation treatment of malignant tumours in the skin. Calcium electroporation is a local treatment that induces supraphysiological intracellular calcium levels by intratumoural calcium administration and application of electrical pulses. The pulses create transient membrane pores allowing diffusion of non-permeant calcium ions into target cells. High calcium levels can kill cancer cells, while normal cells can restore homeostasis. Prior trials with smaller cohorts have found calcium electroporation to be safe and efficient. This trial aims to include a larger multiregional cohort of patients with different cancer diagnoses and also to investigate treatment areas using MRI as well as assess impact on quality of life.Methods and analysisThis non-randomised phase II multicentre study will investigate response to calcium electroporation in 30 patients with cutaneous or subcutaneous malignancy. Enrolment of 10 patients is planned at three centres: Zealand University Hospital, University Hospital of Southern Denmark and University Hospital Schleswig-Holstein. Response after 2 months was chosen as the primary endpoint based on short-term response rates observed in a prior clinical study. Secondary endpoints include response to treatment using MRI and change in quality of life assessed by questionnaires and qualitative interviews.Ethics and disseminationThe trial is approved by the Danish Medicines Agency and The Danish Regional Committee on Health Research Ethics. All included patients will receive active treatment (calcium electroporation). Patients can continue systemic treatment during the study, and side effects are expected to be limited. Data will be published in a peer-reviewed journal and made available to the public.Trial registration numbersNCT04225767 and EudraCT no: 2019-004314-34.

Dataset Information

Calcium electroporation of esophageal cancer induces gene expression changes: a sub-study of a phase I clinical trial.

Purpose

Methods

Results

Conclusion

Publications

Calcium electroporation of esophageal cancer induces gene expression changes: a sub-study of a phase I clinical trial.

Similar Datasets

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