Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis.

ABSTRACT:

Background

The bacterium Bacillus subtilis is extensively used for the commercial production of enzymes due to its efficient protein secretion capacity. However, the efficiency of secretion varies greatly between enzymes, and despite many years of research, optimization of enzyme production is still largely a matter of trial-and-error. Genome-wide transcriptome analysis seems a useful tool to identify relevant secretion bottlenecks, yet to this day, only a limited number of transcriptome studies have been published that focus on enzyme secretion in B. subtilis. Here, we examined the effect of high-level expression of the commercially important enzyme endo-1,4-β-xylanase XynA on the B. subtilis transcriptome using RNA-seq.

Results

Using the novel gene-set analysis tool GINtoo

SUBMITTER: Wang B 

PROVIDER: S-EPMC10633939 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature