Metagenomic discovery of lipases with predicted structural similarity to Candida antarctica lipase B.

ABSTRACT: Here we employed sequence-based and structure-based screening for prospecting lipases that have structural homolog to Candida antarctica lipase B (CalB). CalB, a widely used biocatalyst, was used as structural template reference because of its enzymatic properties. Structural homolog could aid in the discovery of novel wild-type enzymes with desirable features and serve as a scaffold for further biocatalyst design. The available metagenomic data isolated from various environments was leveraged as a source for bioprospecting. We identified two bacteria lipases that showed high structural similarity to CalB with <40% sequence identity. Partial purification was conducted. In comparison to CalB, the enzymatic characteristics of two potential lipases were examined. A candidate exhibited optimal

SUBMITTER: Jaito N 

PROVIDER: S-EPMC10699602 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature