Dataset Information

First report of phenotypic and genotypic (bla_OXA-61) beta-lactam resistance in Campylobacter jejuni from broilers in Indonesia.

ABSTRACT:

Background and aim

Campylobacter is a zoonotic bacterium that is a major source of foodborne diseases. In humans, most cases of campylobacteriosis are caused by Campylobacter jejuni. Poultry is the main reservoir of Campylobacter for humans, because Campylobacter is part of the normal flora of the digestive tract of poultry. Antimicrobial resistance to several antibiotics in Campylobacter isolated from humans and food animals has increased rapidly. Beta-lactam is an antibiotic with a high prevalence of resistance in Campylobacter. This study aimed to investigate phenotypic and genotypic (bla_OXA-61) beta-lactam resistance in C. jejuni from broilers in Indonesia.

Materials and methods

A total of 100 samples of broiler intestinal contents were obtained from 10 broiler farms in Pasuruan Regency, Indonesia. Campylobacter jejuni was identified using conventional and polymerase chain reaction (PCR)-based methods. Phenotypic detection of beta-lactam resistance was performed using an antimicrobial susceptibility test with antibiotic disks of aztreonam, ampicillin, and amoxicillin-clavulanic acid. Genotypic detection by PCR was performed using the bla_OXA-61 gene, which encodes beta-lactamase.

Results

Campylobacter jejuni was identified in 23% of the samples. Phenotypically, 100% (23/23) and 73.9% (17/23) C. jejuni isolates had high resistance to aztreonam and ampicillin, respectively, but all isolates were susceptible to amoxicillin-clavulanic acid. Genotypically, all isolates carried bla_OXA-61, indicated by the presence of a 372-bp PCR product.

Conclusion

Campylobacter jejuni is highly resistant to beta-lactams and is a serious threat to human health. Resistance to beta-lactams should be monitored because beta-lactamase genes can be transferred between bacteria. Public awareness must also be increased on the importance of using antibiotics rationally in humans and animals.

SUBMITTER: Yanestria SM

PROVIDER: S-EPMC10750733 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Publications

First report of phenotypic and genotypic (blaOXA-61) beta-lactam resistance in Campylobacter jejuni from broilers in Indonesia.

Yanestria Sheila Marty SM Effendi Mustofa Helmi MH Tyasningsih Wiwiek W Mariyono Mariyono M Ugbo Emmanuel Nnabuike EN

Veterinary world 20231102 11

<h4>Background and aim</h4>Campylobacter is a zoonotic bacterium that is a major source of foodborne diseases. In humans, most cases of campylobacteriosis are caused by Campylobacter jejuni. Poultry is the main reservoir of Campylobacter for humans, because Campylobacter is part of the normal flora of the digestive tract of poultry. Antimicrobial resistance to several antibiotics in Campylobacter isolated from humans and food animals has increased rapidly. Beta ...[more]

PMID: 38152271

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