Sestrin1, 2, and 3 are dispensable for female fertility in mice.

ABSTRACT:

Background

Sestrins have been implicated in regulating aging in various organs through multiple pathways. However, their roles in ovarian aging remain unrevealed.

Methods

Female Sestrin1^-/-, Sestrin2^-/-, and Sestrin3^-/- mice were generated using the CRISPR-Cas9 system. Body weights, little sizes, ovarian weights, estrous cyclicity, and follicle number in female mice were observed. ELISA was utilized to measure serum anti-Müllerian hormone (AMH) levels. Real time PCR, western blot, immunofluorescence, and Masson trichrome staining were employed for assessment of aging-related change.

Results

The deletion of Sestrin 1, 2, or 3 had no discernible impact on body weights,or serum AMH levels in female mice at the age of 12 months. And there

SUBMITTER: Wang M 

PROVIDER: S-EPMC10832176 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature