HIF1α is dispensable for oocyte development and female fertility in mice.

ABSTRACT:

Background

It has been thought that oocyte may develop in a low oxygen environment, as changes in follicle structure and formation of a fluid-filled antrum. The survival of hypoxic tissues is controlled by hypoxia-inducible factors (HIFs) that are activated in a low oxygen state. HIF1α is expressed in mature mouse oocytes and continues to be expressed after fertilization, from the 2-cell to blastocyst stage. However, the physiological roles of HIF pathway during oogenesis and embryogenesis have still not been elucidated in detail.

Methods

Mutant mice with oocyte-specific HIF1α deletion were generated by crossing Hif1α ^fl/fl mice with transgenic mice expressing Gdf9-promoter-mediated Cre recombinase. Breeding assay w

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9074875 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature