High pressure pasteurization: Simultaneous native tissue decellularization and sterilization.

ABSTRACT:

Introduction

Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated. This study investigated the disinfection efficacy of the HHP process at physiologically relevant 36 °C against difficult-to-kill spore-forming bacteria.

Methods

Bacillus atrophaeus and Geobacillus stearothermophilus were suspended in a pressurization medium with or without antibiotic agents and pressurized under two different HHP procedures: repeated and sustained pressurization.

Results

The sustained pressurizing conditions, exploited for the conventional ti

SUBMITTER: Otaka A 

PROVIDER: S-EPMC10864870 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature