Phosphoinositide acyl chain saturation drives CD8⁺ effector T cell signaling and function.

ABSTRACT: How lipidome changes support CD8⁺ effector T (T_eff) cell differentiation is not well understood. Here we show that, although naive T cells are rich in polyunsaturated phosphoinositides (PIP_n with 3-4 double bonds), T_eff cells have unique PIP_n marked by saturated fatty acyl chains (0-2 double bonds). PIP_n are precursors for second messengers. Polyunsaturated phosphatidylinositol bisphosphate (PIP₂) exclusively supported signaling immediately upon T cell antigen receptor activation. In late T_eff cells, activity of phospholipase C-γ1, the enzyme that cleaves PIP₂ into downstream mediators, waned, and saturated PIP_n became essential for sustained signaling. Saturated PIP was more rapid

SUBMITTER: Edwards-Hicks J 

PROVIDER: S-EPMC10908374 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature