Validating Enteroid-Derived Monolayers from Murine Gut Organoids for Toxicological Testing of Inorganic Particles: Proof-of-Concept with Food-Grade Titanium Dioxide.

ABSTRACT: Human exposure to foodborne inorganic nanoparticles (NPs) is a growing concern. However, identifying potential hazards linked to NP ingestion often requires long-term exposure in animals. Owing these constraints, intestinal organoids are a promising alternative to in vivo experiments; as such, an in vitro approach should enable a rapid and reliable assessment of the effects of ingested chemicals on the gut. However, this remains to be validated for inorganic substances. In our study, a transcriptomic analysis and immunofluorescence staining were performed to compare the effects of food-grade TiO₂ (fg-TiO₂) on enteroid-derived monolayers (EDMs) from murine intestinal organoids to the known impacts of TiO₂ on intestinal epithelium. After their ability

SUBMITTER: Malaise Y 

PROVIDER: S-EPMC10932210 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature