Dataset Information

Identification of macrophage-related genes correlated with prognosis and immunotherapy efficacy in non-small cell lung cancer.

ABSTRACT:

Background

Malignant tumours, particularly non-small cell lung cancer (NSCLC), pose a significant threat to human health due to their prevalence and lethality. Treatment methods for NSCLC vary greatly among individuals, making it crucial to identify predictive markers. Moreover, during tumour initiation and progression, tumour cells can release signaling molecules to induce polarization of macrophages towards a more tumour friendly M2 phenotype, which can promote tumour growth, metastasis, and drug resistance.

Methods

We employed a comprehensive approach, combining bulk RNA-seq and single-cell sequencing analysis.

Results

In our study, we used bulk RNA-seq and single-cell sequencing methods to analyze differential cells in NSCLC and adjacent tissues, searching for relevant marker genes that can predict prognosis and drug efficacy. We scrutinized biological phenomena such as macrophage-related gene methylation, copy number variation, and alternative splicing. Additionally, we utilized a co-culture technique of immune and tumour cells to explore the role of these genes in macrophage polarization. Our findings revealed distinct differences in macrophages between cancerous and adjacent tissues. We identified ANP32A, CCL20, ERAP2, MYD88, TMEM126B, TUBB6, and ZNF655 as macrophage-related genes that correlate with NSCLC patient prognosis and immunotherapy efficacy. Notably, ERAP2, TUBB6, CCL20, and TMEM126B can induce macrophage M0 to M2 polarization, promoting tumour proliferation.

Conclusion

These findings significantly contribute to our understanding of the NSCLC tumour immune microenvironment. They pave the way for further research into the potential of these genes as targets for regulating tumour occurrence and development.

SUBMITTER: Wen S

PROVIDER: S-EPMC10945138 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Publications

Identification of macrophage-related genes correlated with prognosis and immunotherapy efficacy in non-small cell lung cancer.

Wen Shaodi S Zou Renrui R Du Xiaoyue X Pan Rongtian R Li Rutao R Xia Jingwei J Xu Cong C Wang Ruotong R Jiang Feng F Zhou Guoren G Feng Jifeng J Zhu Miaolin M Wang Xin X Shen Bo B

Heliyon 20240307 6

<h4>Background</h4>Malignant tumours, particularly non-small cell lung cancer (NSCLC), pose a significant threat to human health due to their prevalence and lethality. Treatment methods for NSCLC vary greatly among individuals, making it crucial to identify predictive markers. Moreover, during tumour initiation and progression, tumour cells can release signaling molecules to induce polarization of macrophages towards a more tumour friendly M2 phenotype, which can promote tumour growth, metastasi ...[more]

PMID: 38500993

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Dataset Information

Identification of macrophage-related genes correlated with prognosis and immunotherapy efficacy in non-small cell lung cancer.

Background

Methods

Results

Conclusion

Publications

Identification of macrophage-related genes correlated with prognosis and immunotherapy efficacy in non-small cell lung cancer.

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