A novel Na_v1.8-FLPo driver mouse for intersectional genetics to uncover the functional significance of primary sensory neuron diversity.

ABSTRACT: The recent development of single-cell and single-nucleus RNA sequencing has highlighted the extraordinary diversity of dorsal root ganglia neurons. However, the few available genetic tools limit our understanding of the functional significance of this heterogeneity. We generated a new mouse line expressing the flippase recombinase from the scn10a locus. By crossing Na_v1.8^Ires-FLPo mice with the Advillin^Cre and RC::FL-hM3Dq mouse lines in an intersectional genetics approach, we were able to obtain somatodendritic expression of hM3Dq-mCherry selectively in the Na_v1.8 lineage. The bath application of clozapine N-oxide triggered strong calcium responses selectively in mCherry⁺ neurons. The intraplantar injection of CNO caused robust

SUBMITTER: Malapert P 

PROVIDER: S-EPMC10952036 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature