Increased Neutrophil H₂O₂ Production and Enhanced Pulmonary Clearance of Klebsiella pneumoniae in G6PD A- Mice.

ABSTRACT: The X-linked A^- variant (rs1050828, Val68Met) in G6PDX accounts for glucose-6-phosphate (G6PD) deficiency in approximately 11% of African American males. This common, hypomorphic variant may impact pulmonary host defense and phagocyte function during pneumonia by altering levels of reactive oxygen species produced by host leukocytes. We used CRISPR-Cas9 technology to generate novel mouse strain with "humanized" G6PD A- variant containing non-synonymous Val68Met single nucleotide polymorphism. Male hemizygous or littermate wild-type (WT) controls were inoculated intratracheally with K. pneumoniae (KP2 serotype, ATCC 43816 strain,10³ CFU inoculum). We examined leukocyte recruitment, organ bacterial burden, bone marrow neutrophil and macrophage (BMDM) phago

SUBMITTER: Zuchelkowski BE 

PROVIDER: S-EPMC10980108 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature