Universal recording of immune cell interactions in vivo.

ABSTRACT: Immune cells rely on transient physical interactions with other immune and non-immune populations to regulate their function¹. To study these 'kiss-and-run' interactions directly in vivo, we previously developed LIPSTIC (labelling immune partnerships by SorTagging intercellular contacts)², an approach that uses enzymatic transfer of a labelled substrate between the molecular partners CD40L and CD40 to label interacting cells. Reliance on this pathway limited the use of LIPSTIC to measuring interactions between CD4⁺ T helper cells and antigen-presenting cells, however. Here we report the development of a universal version of LIPSTIC (uLIPSTIC), which can record physical interactions both among immune cells and between immune and non-immune populations irresp

SUBMITTER: Nakandakari-Higa S 

PROVIDER: S-EPMC11078586 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature