CRISPR-Cas3 and type I restriction-modification team up against bla_KPC-IncF plasmid transfer in Klebsiella pneumoniae.

ABSTRACT:

Objective

We explored whether the Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas and restriction-modification (R-M) systems are compatible and act together to resist plasmid attacks.

Methods

932 global whole-genome sequences from GenBank, and 459 K. pneumoniae isolates from six provinces of China, were collected to investigate the co-distribution of CRISPR-Cas, R-M systems, and bla_KPC plasmid. Conjugation and transformation assays were applied to explore the anti-plasmid function of CRISPR and R-M systems.

Results

We found a significant inverse correlation between the presence of CRISPR and R-M systems and bla_KPC plasmids in K. pneumoniae, especially when both systems cohabited in one host. The multiple matched recognition

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11223367 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature