Dataset Information

Pilot Evaluation of S-(3-[¹⁸F]Fluoropropyl)-D-Homocysteine and O-(2-[¹⁸F]Fluoroethyl)-D-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection.

ABSTRACT:

Purpose

There is currently no ideal radiotracer for imaging bacterial infections. Radiolabelled D-amino acids are promising candidates because they are actively incorporated into the peptidoglycan of the bacterial cell wall, a structural feature which is absent in human cells. This work describes fluorine-18 labelled analogues of D-tyrosine and D-methionine, O-(2-[¹⁸F]fluoroethyl)-D-tyrosine (D-[¹⁸F]FET) and S-(3-[¹⁸F]fluoropropyl)-D-homocysteine (D-[¹⁸F]FPHCys), and their pilot evaluation studies as potential radiotracers for imaging bacterial infection.

Procedures

D-[¹⁸F]FET and D-[¹⁸F]FPHCys were prepared in classical fluorination-deprotection reactions, and their uptake in Staphylococcus aureus and Pseudomonas aeruginosa was evaluated over 2 h. Heat killed bacteria were used as controls. A clinically-relevant foreign body model of S. aureus infection was established in Balb/c mice, as well as a sterile foreign body to mimic inflammation. The ex vivo biodistribution of D-[¹⁸F]FPHCys in the infected and inflamed mice was evaluated after 1 h, by dissection and gamma counting. The uptake was compared to that of [¹⁸F]FDG.

Results

In vitro uptake of both D-[¹⁸F]FET and D-[¹⁸F]FPHCys was specific to live bacteria. Uptake was higher in S. aureus than in P. aeruginosa for both radiotracers, and of the two, higher for D-[¹⁸F]FPHCys than D-[¹⁸F]FET. Blocking experiments with non-radioactive D-[¹⁹F]FPHCys confirmed specificity of uptake. In vivo, D-[¹⁸F]FPHCys had greater accumulation in S. aureus infection compared with sterile inflammation, which was statistically significant. As anticipated, [¹⁸F]FDG showed no significant difference in uptake between infection and inflammation.

Conclusions

D-[¹⁸F]FPHCys uptake was higher in infected tissues than inflammation, and represents a fluorine-18 labelled D-AA with potential to detect a S. aureus reference strain (Xen29) in vivo. Additional studies are needed to evaluate uptake of this radiotracer in clinical isolates.

SUBMITTER: Betts HM

PROVIDER: S-EPMC11282134 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Pilot Evaluation of S-(3-[18F]Fluoropropyl)-D-Homocysteine and O-(2-[18F]Fluoroethyl)-D-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection.

Betts Helen M HM Luckett Jeni C JC Hill Philip J PJ

Molecular imaging and biology 20240628 4

<h4>Purpose</h4>There is currently no ideal radiotracer for imaging bacterial infections. Radiolabelled D-amino acids are promising candidates because they are actively incorporated into the peptidoglycan of the bacterial cell wall, a structural feature which is absent in human cells. This work describes fluorine-18 labelled analogues of D-tyrosine and D-methionine, O-(2-[18F]fluoroethyl)-D-tyrosine (D-[18F]FET) and S-(3-[18F]fluoropropyl)-D-homocysteine (D-[<sup ...[more]

PMID: 38942967

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