Metabolic Choreography of Energy Substrates During DCD Heart Perfusion.

ABSTRACT:

Background

The number of patients waiting for heart transplant far exceeds the number of hearts available. Donation after circulatory death (DCD) combined with machine perfusion can increase the number of transplantable hearts by as much as 48%. Emerging studies also suggest machine perfusion could enable allograft "reconditioning" to optimize outcomes. However, a detailed understanding of the energetic substrates and metabolic changes during perfusion is lacking.

Methods

Metabolites were analyzed using 1-dimensional ¹H and 2-dimensional ¹³C-¹H heteronuclear spectrum quantum correlation nuclear magnetic resonance spectroscopy on serial perfusate samples (N = 98) from 32 DCD hearts that were successfully transplanted. Wilcoxon signed-rank and

SUBMITTER: Trimigno A 

PROVIDER: S-EPMC11365673 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature