An updated phylogeny of Boraginales based on the Angiosperms353 probe set: a roadmap for understanding morphological evolution.

ABSTRACT:

Background and aims

Boraginales, a subcosmopolitan order with ~2700 species in currently 11 families, has seen fluctuating classification at the family and generic levels. Gynoecium and fruit morphology have been pivotal in morphological classifications, but a systematic evaluation based on anatomical and ontogenetic data has not been attempted.

Methods

We revisited the phylogenetic relationships in Boraginales by analysing 162 newly sequenced samples using the Angiosperms353 probe set, together with existing data from 88 samples. Our sampling covers >80 % of the genera and all currently and previously recognized families. A morphological assessment of taxonomically important gynoecial and fruit characters was done to guide our proposed family-level classification.

Key results

The phylogenies are largely consistent with previous phylogenetic studies, with better resolution overall and support from both datasets (exons, supercontigs) and all analyses implemented (coalescence, ASTRAL-Pro3, concatenation). The placement of Hydrophyllaceae as nested within Namaceae is a novel result, while the placement of the parasitic Lennoaceae as nested within Ehretiaceae is confirmed with maximum confidence. The placement of Codonaceae remains ambiguous: retrieved within Boraginales I, as sister to the clade comprising Wellstediaceae and Boraginaceae based on exons, and as sister branch to all remaining Boraginales II based on supercontigs. We propose the recognition of a total of nine families in Boraginales. Tribal relationships in Boraginaceae subfam. Cynoglossoideae are fully resolved for the first time. Our data show that shifts in placentation and ovule number are instrumental for the arisal of complex internal ovary architectures.

Conclusions

The results highlight the effectiveness of the Angiosperms353 probe set for understanding the evolutionary history of Boraginales and pave the way for resolving remaining unresolved nodes and taxonomic issues. Complex modifications of bicarpellate ovaries led to an extreme and lineage-specific diversification of fruits that probably played a crucial role in Boraginales macroevolution. Combining this highly resolved phylogeny with future critical morphological analyses promises a deep understanding of evolutionary trajectories in Boraginales.

SUBMITTER: Vasile MA 

PROVIDER: S-EPMC12401892 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature