Comparative genomic analysis of Mycobacterium avium subspecies obtained from multiple host species.

ABSTRACT:

Background

Mycobacterium avium (M. avium) subspecies vary widely in both pathogenicity and host specificity, but the genetic features contributing to this diversity remain unclear.

Results

A comparative genomic approach was used to identify large sequence polymorphisms among M. avium subspecies obtained from a variety of host animals. DNA microarrays were used as a platform for comparing mycobacterial isolates with the sequenced bovine isolate M. avium subsp. paratuberculosis (MAP) K-10. Open reading frames (ORFs) were classified as present or divergent based on the relative fluorescent intensities of the experimental samples compared to MAP K-10 DNA. Multiple large polymorphic regions were found in the genomes of MAP isolates obtained from sheep. One of these clusters encod

SUBMITTER: Paustian ML 

PROVIDER: S-EPMC2323391 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature