Site-specific GlcNAcylation of human erythrocyte proteins: potential biomarker(s) for diabetes.

ABSTRACT:

Objective

O-linked N-acetylglucosamine (O-GlcNAc) is upregulated in diabetic tissues and plays a role in insulin resistance and glucose toxicity. Here, we investigated the extent of GlcNAcylation on human erythrocyte proteins and compared site-specific GlcNAcylation on erythrocyte proteins from diabetic and normal individuals.

Research design and methods

GlcNAcylated erythrocyte proteins or GlcNAcylated peptides were tagged and selectively enriched by a chemoenzymatic approach and identified by mass spectrometry. The enrichment approach was combined with solid-phase chemical derivatization and isotopic labeling to detect O-GlcNAc modification sites and to compare site-specific O-GlcNAc occupancy levels between normal and diabetic erythrocyte proteins.

Results

The enzy

SUBMITTER: Wang Z 

PROVIDER: S-EPMC2628603 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature