Synthesizing non-natural parts from natural genomic template.

ABSTRACT:

Background

The current knowledge of genes and proteins comes from 'naturally designed' coding and non-coding regions. It would be interesting to move beyond natural boundaries and make user-defined parts. To explore this possibility we made six non-natural proteins in E. coli. We also studied their potential tertiary structure and phenotypic outcomes.

Results

The chosen intergenic sequences were amplified and expressed using pBAD 202/D-TOPO vector. All six proteins showed significantly low similarity to the known proteins in the NCBI protein database. The protein expression was confirmed through Western blot. The endogenous expression of one of the proteins resulted in the cell growth inhibition. The growth inhibition was completely rescued by culturing cells in the inducer-

SUBMITTER: Dhar PK 

PROVIDER: S-EPMC2642765 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature