Genomic-wide analysis of lymphatic metastasis-associated genes in human hepatocellular carcinoma.

ABSTRACT:

Aim

To identify the genes related to lymph node metastasis in human hepatocellular carcinoma (HCC), 32 HCC patients with or without lymph node metastasis were investigated by high-throughput microarray comprising 886 genes.

Methods

The samples of cancerous and non-cancerous paired tissue were taken from 32 patients with HCC who underwent hepatectomy with lymph node dissection. Total RNA was extracted from the cells obtained by means of laser microdissection (LCM) and was amplified by the T7-based amplification system. Then, the amplified samples were applied in the cDNA microarray comprising of 886 genes.

Results

The results demonstrated that 25 up-regulated genes such as cell membrane receptor, intracellular signaling and cell adhesion related genes, and 48 down-regu

SUBMITTER: Lee CF 

PROVIDER: S-EPMC2653334 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature