DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR.

ABSTRACT:

Background

The TetR family member AmtR is the central regulator of nitrogen starvation response in Corynebacterium glutamicum. While the AmtR regulon was physiologically characterized in great detail up to now, mechanistic questions of AmtR binding were not addressed. This study presents a characterization of functionally important amino acids in the DNA binding domain of AmtR and of crucial nucleotides in the AmtR recognition motif.

Results

Site-directed mutagenesis, the characterization of corresponding mutant proteins by gel retardation assays and surface plasmon resonance and molecular modelling revealed several amino acids, which are directly involved in DNA binding, while others have more structural function. Furthermore, we could show that the spacing of the binding m

SUBMITTER: Muhl D 

PROVIDER: S-EPMC2723105 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature