Glycan family analysis for deducing N-glycan topology from single MS.

ABSTRACT:

Motivation

In the past few years, mass spectrometry (MS) has emerged as the premier tool for identification and quantification of biological molecules such as peptides and glycans. There are two basic strategies: single-MS, which uses a single round of mass analysis, and MS/MS (or higher order MS(n)), which adds one or more additional rounds of mass analysis, interspersed with fragmentation steps. Single-MS offers higher throughput, broader mass coverage and more direct quantitation, but generally much weaker identification. Single-MS, however, does work fairly well for the case of N-glycan identification, which are more constrained than other biological polymers. We previously demonstrated single-MS identification of N-glycans to the level of 'cartoons' (monosaccharide composition

SUBMITTER: Goldberg D 

PROVIDER: S-EPMC2732296 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature