Age, obesity, and sex effects on insulin sensitivity and skeletal muscle mitochondrial function.

ABSTRACT:

Objective

Reductions in insulin sensitivity in conjunction with muscle mitochondrial dysfunction have been reported to occur in many conditions including aging. The objective was to determine whether insulin resistance and mitochondrial dysfunction are directly related to chronological age or are related to age-related changes in body composition.

Research design and methods

Twelve young lean, 12 young obese, 12 elderly lean, and 12 elderly obese sedentary adults were studied. Insulin sensitivity was measured by a hyperinsulinemic-euglycemic clamp, and skeletal muscle mitochondrial ATP production rates (MAPRs) were measured in freshly isolated mitochondria obtained from vastus lateralis biopsy samples using the luciferase reaction.

Results

Obese participants, independ

SUBMITTER: Karakelides H 

PROVIDER: S-EPMC2797949 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature